human syn proteins Search Results


synthetic peptide  (Aviva Systems)
90
Aviva Systems synthetic peptide
Synthetic Peptide, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ubiquitin vinyl sulfone  (Boston Biochem)
90
Boston Biochem ubiquitin vinyl sulfone
Figure 2. UAF1 Stabilizes USP1 In Vivo and In Vitro (A) HeLa cells stably expressing shRNA plas- mid contructs targeting UAF1 or a control shRNA plasmid. Protein lysates were pre- pared, and the samples were analyzed by im- munoblotting using the indicated antibodies. (B) Coomassie blue stain of Flag-HA-tagged UAF1 protein purified from HeLa cells. (C) c-Myc-tagged USP1 was synthesized in rabbit reticulocyte either in the absence or presence of UAF1 protein. Translation was in- hibited by the addition of cyclohexamide after 1 hr, and the reactions were continued for the indicated times. The reactions were analyzed by immunoblotting using anti-c-Myc (top) or anti-UAF1 antibodies (bottom). (D) c-Myc-tagged USP1 was synthesized as in (C). Cyclohexamide was added after 1 hr and <t>Ub-VS</t> after another 2 hr. The reactions were then allowed to continue for 2 hr before they were terminated and analyzed by immu- noblotting using the same antibodies as in (C). (E) Both full-length and the N-terminal cleavage product of USP1 possess deubiquitinating enzyme activity. The native USP1 complex pu- rified from HeLa cells, or a mock purification, was subjected to an in vitro Ub-VS assay for 2 hr.
Ubiquitin Vinyl Sulfone, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adeno-associated virus (aav) targeting to knockdown the entpd1 gene by shrna-entpd1 via cre-loxp strategy  (BrainVTA (Wuhan) Co Ltd)
90
BrainVTA (Wuhan) Co Ltd adeno-associated virus (aav) targeting to knockdown the entpd1 gene by shrna-entpd1 via cre-loxp strategy
Figure 2. UAF1 Stabilizes USP1 In Vivo and In Vitro (A) HeLa cells stably expressing shRNA plas- mid contructs targeting UAF1 or a control shRNA plasmid. Protein lysates were pre- pared, and the samples were analyzed by im- munoblotting using the indicated antibodies. (B) Coomassie blue stain of Flag-HA-tagged UAF1 protein purified from HeLa cells. (C) c-Myc-tagged USP1 was synthesized in rabbit reticulocyte either in the absence or presence of UAF1 protein. Translation was in- hibited by the addition of cyclohexamide after 1 hr, and the reactions were continued for the indicated times. The reactions were analyzed by immunoblotting using anti-c-Myc (top) or anti-UAF1 antibodies (bottom). (D) c-Myc-tagged USP1 was synthesized as in (C). Cyclohexamide was added after 1 hr and <t>Ub-VS</t> after another 2 hr. The reactions were then allowed to continue for 2 hr before they were terminated and analyzed by immu- noblotting using the same antibodies as in (C). (E) Both full-length and the N-terminal cleavage product of USP1 possess deubiquitinating enzyme activity. The native USP1 complex pu- rified from HeLa cells, or a mock purification, was subjected to an in vitro Ub-VS assay for 2 hr.
Adeno Associated Virus (Aav) Targeting To Knockdown The Entpd1 Gene By Shrna Entpd1 Via Cre Loxp Strategy, supplied by BrainVTA (Wuhan) Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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α-syn  (Human Protein Atlas)
90
Human Protein Atlas α-syn
Figure 2. UAF1 Stabilizes USP1 In Vivo and In Vitro (A) HeLa cells stably expressing shRNA plas- mid contructs targeting UAF1 or a control shRNA plasmid. Protein lysates were pre- pared, and the samples were analyzed by im- munoblotting using the indicated antibodies. (B) Coomassie blue stain of Flag-HA-tagged UAF1 protein purified from HeLa cells. (C) c-Myc-tagged USP1 was synthesized in rabbit reticulocyte either in the absence or presence of UAF1 protein. Translation was in- hibited by the addition of cyclohexamide after 1 hr, and the reactions were continued for the indicated times. The reactions were analyzed by immunoblotting using anti-c-Myc (top) or anti-UAF1 antibodies (bottom). (D) c-Myc-tagged USP1 was synthesized as in (C). Cyclohexamide was added after 1 hr and <t>Ub-VS</t> after another 2 hr. The reactions were then allowed to continue for 2 hr before they were terminated and analyzed by immu- noblotting using the same antibodies as in (C). (E) Both full-length and the N-terminal cleavage product of USP1 possess deubiquitinating enzyme activity. The native USP1 complex pu- rified from HeLa cells, or a mock purification, was subjected to an in vitro Ub-VS assay for 2 hr.
α Syn, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human α-syn protein  (Brookhaven Instruments)
90
Brookhaven Instruments human α-syn protein
Figure 2. UAF1 Stabilizes USP1 In Vivo and In Vitro (A) HeLa cells stably expressing shRNA plas- mid contructs targeting UAF1 or a control shRNA plasmid. Protein lysates were pre- pared, and the samples were analyzed by im- munoblotting using the indicated antibodies. (B) Coomassie blue stain of Flag-HA-tagged UAF1 protein purified from HeLa cells. (C) c-Myc-tagged USP1 was synthesized in rabbit reticulocyte either in the absence or presence of UAF1 protein. Translation was in- hibited by the addition of cyclohexamide after 1 hr, and the reactions were continued for the indicated times. The reactions were analyzed by immunoblotting using anti-c-Myc (top) or anti-UAF1 antibodies (bottom). (D) c-Myc-tagged USP1 was synthesized as in (C). Cyclohexamide was added after 1 hr and <t>Ub-VS</t> after another 2 hr. The reactions were then allowed to continue for 2 hr before they were terminated and analyzed by immu- noblotting using the same antibodies as in (C). (E) Both full-length and the N-terminal cleavage product of USP1 possess deubiquitinating enzyme activity. The native USP1 complex pu- rified from HeLa cells, or a mock purification, was subjected to an in vitro Ub-VS assay for 2 hr.
Human α Syn Protein, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. UAF1 Stabilizes USP1 In Vivo and In Vitro (A) HeLa cells stably expressing shRNA plas- mid contructs targeting UAF1 or a control shRNA plasmid. Protein lysates were pre- pared, and the samples were analyzed by im- munoblotting using the indicated antibodies. (B) Coomassie blue stain of Flag-HA-tagged UAF1 protein purified from HeLa cells. (C) c-Myc-tagged USP1 was synthesized in rabbit reticulocyte either in the absence or presence of UAF1 protein. Translation was in- hibited by the addition of cyclohexamide after 1 hr, and the reactions were continued for the indicated times. The reactions were analyzed by immunoblotting using anti-c-Myc (top) or anti-UAF1 antibodies (bottom). (D) c-Myc-tagged USP1 was synthesized as in (C). Cyclohexamide was added after 1 hr and Ub-VS after another 2 hr. The reactions were then allowed to continue for 2 hr before they were terminated and analyzed by immu- noblotting using the same antibodies as in (C). (E) Both full-length and the N-terminal cleavage product of USP1 possess deubiquitinating enzyme activity. The native USP1 complex pu- rified from HeLa cells, or a mock purification, was subjected to an in vitro Ub-VS assay for 2 hr.

Journal: Molecular cell

Article Title: A UAF1-containing multisubunit protein complex regulates the Fanconi anemia pathway.

doi: 10.1016/j.molcel.2007.09.031

Figure Lengend Snippet: Figure 2. UAF1 Stabilizes USP1 In Vivo and In Vitro (A) HeLa cells stably expressing shRNA plas- mid contructs targeting UAF1 or a control shRNA plasmid. Protein lysates were pre- pared, and the samples were analyzed by im- munoblotting using the indicated antibodies. (B) Coomassie blue stain of Flag-HA-tagged UAF1 protein purified from HeLa cells. (C) c-Myc-tagged USP1 was synthesized in rabbit reticulocyte either in the absence or presence of UAF1 protein. Translation was in- hibited by the addition of cyclohexamide after 1 hr, and the reactions were continued for the indicated times. The reactions were analyzed by immunoblotting using anti-c-Myc (top) or anti-UAF1 antibodies (bottom). (D) c-Myc-tagged USP1 was synthesized as in (C). Cyclohexamide was added after 1 hr and Ub-VS after another 2 hr. The reactions were then allowed to continue for 2 hr before they were terminated and analyzed by immu- noblotting using the same antibodies as in (C). (E) Both full-length and the N-terminal cleavage product of USP1 possess deubiquitinating enzyme activity. The native USP1 complex pu- rified from HeLa cells, or a mock purification, was subjected to an in vitro Ub-VS assay for 2 hr.

Article Snippet: In vitro enzymatic assays in the presence of 5 mM ubiquitin vinyl sulfone (Ub-VS; U-202; Boston Biochem) were performed at 30 C. In vitro enzymatic assays using ubiquitin-7-amido-4-methylcoumarin (Ub-AMC; U-550; Boston Biochem) were performed in 50–100 ml reaction buffer (20 mM HEPES-KOH [pH 7.8], 20 mM NaCl, 0.1 mg/ml ovalbumin [A7641; Sigma], 0.5 mM EDTA, and 10 mM DTT) at 37 C. Fluorescence was monitored in a FluoStar Galaxy Fluorometer (BMG Labtech).

Techniques: In Vivo, In Vitro, Stable Transfection, Expressing, shRNA, Control, Plasmid Preparation, Staining, Synthesized, Western Blot, Activity Assay